Spectrophotometric Assay of Iron (II) in Pharmaceutical Formulation Using Alizarin Red Sulphonate Reagent


AbstractA rapid, sensitive and simple spectrophotometric method was developed for the determination of iron (II). The method was based on the reaction of iron (II) with alizarin red sulphonate reagent to form a brown chelating complex in an aqueous solution. The absorbance of the chelating complex was measured at 566 nm with a molar absorptivity of 7.8 x 103 l.mol-1.cm-1. The chelating complex conforms to Beer's law over the range (0.5-5) g.ml-1. the average recovery% was 100.06% and precision (RSD) was found to be less than 1%. The method was successfully employed for assay of iron (II) in pharmaceutical formulations (tablets and capsules). The results have been compared with British pharmacopoeia method.