Anti-rotavirus IgG Seropositivity among Healthy Population and Patients with Acute Diarrhea in Baquba-Diyala Province


Background: Rotavirus infection is the leading single cause of severe diarrhea among infants and young children. More than 500,000 children under 5 years of age die from rotavirus infection each year, and almost 2 million more become severely ill. Objectives: To determine the rate of anti-rotavirus IgG antibody among healthy population and patients with diarrhea in Baquba- Diyala province. Materials & Methods: The present study was extended from the first of July 2008 to the first of September/ 2009 in Baquba City. A total of 300 fecal specimens were collected from patients suffering from acute diarrhea. The patients include 136 (45.3%) females with mean age (7.8 ± 4.7) years, and 164 males with mean age (5.3± 3.4) years. Biorad-Rota kit is a highly sensitive agglutination test was used for detection of rotavirus in fecal specimens. Anti-rotavirus IgG antibody was detected by ELISA technique. All data were statistically analyzed Results: The results revealed that the anti-rotavirus IgG antibody positivity rate among patients was 49.3% compared to 37.1% among the healthy population. Additionally, 25.7% of patients who were positive for rotavirus infection as detected by agglutination test in the stool were also positive for anti-rotavirus IgG antibody. On the other hand, 17(22.4%) of patients with rotavirus diarrhea were negative for anti-rotavirus IgG antibody. The effects of age, gender, residence, type of water supply, and type of feeding on the frequency of anti-rotavirus IgG antibody in both patients and healthy groups were statistically insignificant.Conclusion: Nearly two third of healthy population in Baquba city are liable for rotavirus infection as they lack anti-rotavirus IgG antibody.Keywords: Rotavirus, acute diarrhea, Anti-rotavirus IgG.