Effect of 650 nm Diode Laser on the Cross-Bonding Formation of Human RBC Membrane


Background & Objective(s): - The current study was designed to study the effect of diode laser on red blood cell membrane protein indirectly assessed (by membrane cross bonding formation).Materials & Methods: - The packed cells were washed three times in M300, and then were resuspended in M600 at an approximate haematocrit of 2%. The RBCs suspension was divided into four equal aliquots. One aliquot was served as control. The remaining three aliquots were irradiated by laser for 30, 40 and 60 min. after each specific time, the control and the irradiated samples were centrifuged and the harvested RBCs were washed once in M200 and were resuspended at an approximate haematocrit of 0.2% in M200. The percentage of cross bonded cells was determined microscopically in each sample as described later.Results: - It was found that the exposure of red blood cells to the laser for 30 min didn’t induce membrane cross- bonded formation. However, prolongation of the irradiation time for 40 min and 60 min was associated with a significant increase in the percentage of cross bonded cells.Conclusion:- exposure of red blood cells to low level diode laser (λ= 650 nm, w= 50 mW) for long period of time equal or more than 40 min led to denaturation of the membrane protein of red blood cells. Keywords:- Laser + Red Blood Cells +Membrane Protein + Cross-Bonding