Microorganisms isolated from Cases of Urinary Tract Infections

Abstract

Background: Escherichia coli reported to be the most common bacteria isolate from patients with UTI, while Klebsilla spp. were found to be the second common isolated, followed by Proteus spp. .Most isolates were highly sensitive to gentamycin, amikacin, cefotaxim, and ciprofloxacin, neither isolate showed susceptibility to tetracycline. Objectives: To isolate microorganisms from patients with UTI, also to determine the susceptibility of the isolates to the commonly used antimicrobial agents. Methods: One hundred and seventy patients with UTI, including 105 females and 65 males with a mean age of 21 years (range 6-54), were enrolled in a study based at Al-Yarmouk Teaching Hospital from June 2008 to May 2009.The urine specimens were immediately analyzed for microorganisms. All isolates were diagnosed according to bacteriological and biochemical standard methods, also using API 20E system and to determine the susceptibility of isolates to antibiotics. Results: Enterobacteriaceae was the most frequently isolate, representing (79.4%), while other isolates showed Gram – positive Staphylococcus aureus(10.2%),Pseudomonas aeruginosa(6.4%) and Fungi(3.9%).Escherichia coli was the most common bacteria isolate (37.1%). Klebsiella spp. were found to be the second common isolate(30.7%), follo -wed by Proteus spp.(16.9%).The other isolates which were Citrobacter spp. (6.4%), Enterobacter spp.(4.0%), Providencia rettegeri (3.2%), and Morganella morganii(1.6%).Most isolates were the highly sensitive to gentamycin, amikacin, cefotaxim and ciprofloxacin. In contrast tetracycline were completely resistant against all isolates.Conclusions: Gram – negative bacilli were the most frequently isolates than Gram-positive cocci in patients with UTI(85.8% vs. 10.2%).All Enterobacteriaceae isolates were complete resistant to tetracycline, thus should not be considered the drug of choice in the treatment of patients with UTI.Key words: Microorganisms, Urinary Infections, Drug resistance.