Evaluation of the Genetic Diversity in Iraqi Local Breeds of Goat Using Random Amplified Polymorphic DNA Markers

Abstract

In the current study, random amplified polymorphic DNA polymerase (RAPD) was used to investigate the genetic diversity and relationship among three breeds of local goat (Black, Brown and White). Twelve individual were subjected to blood samples collection, four from each population. Extracted DNA was in adequate quantity and quality. The polymorphism varied between primers and within breeds. Seven out of sixteen RAPD primers were found to be polymorphic. The maximal number of scorable fragments was amplified by D15, while C5 and C12 primers were shared the same minimal value of amplified fragments which was 8 bands. Molecular size of amplified fragments ranged from 0.16 kbp to 4.8 kbp. Of the 69 amplified fragments, 32 fragments (46%) were polymorphic with an average of 4.57 polymorphic fragments per primer. Dendrogram derived from RAPD profiles separated the twelve individuals into four main clusters; two were consisted of single individual, whereas the other two clusters consisted of three and seven individuals, respectively. Genetic similarity was ranged from 0.61% to 0.97%. The results indicated that RAPD-PCR technique was a valuable tool in the arsenal of animal breeders and can efficiently distinguish between goat breeds.