عزل الجراثيم وتشخيصها من حالات التهاب المجاري البولية واختبار حساسيتها للمضادات الحيوية

Abstract

This study includes the isolation and identification of gram negative and gram positive bacteria causing urinary tract infections,Three hundred twenty four of urine samples were collected from children aged one day to 16 years old in Al- Hawija district for the period 1 st of August 2006 to 30 th April 2007 . The results showed a significant bacterial growth in 177 urine samples 54.6% , forty one cases were males (33.3% ),while 136 cases were females (63.4%) .Isolates were identified according to physical, microscopical, biochemical tests and by APi 20 E system .For gram negative bacteria Escherichia coli was the common isolate (51.4%) folloused by Klebsiella was (27.7%) , Proteus vulgaris 6.2% Proteus mirabilis(5.7%), Citrobacter (1.1%), Pseudomonas and Enterobacter represented (0.6%)for each of them .While for gram positive bacteria Staphylococcis. aureus represent (1.7%), and Coagulase negative Staphylococcus was (5%) . Microscopical test was conducted for the patients urine samples and it has been found that 18.6% of urine samples contain pus cells , 9.6% crystal , 7.9% epithelial cells, 0.6% cast , 1.7% albumin, 4%, bacteria and 3.4% RBCs The isolates were tests for antibiotic sensitivity using 16 antibiotics ,some of them were commonly used for the treatment of urinary tract infection ,while others were prohibited for children , the isolates showed high resistance against betalactam antibiotics (Pencillins) , while most of isolates were sensitive to Quinolones .