ISOLATION AND DETERMINATION OF PROTEIN FRACTIONS OF Trichophyton mentagrophytes Var.erinacei USING GEL FILTERATION CHROMATOGRAPHY

Abstract

The protein in broken-cell extract from Trichophyton mentagrophytes var.erinacei was isolated and fractionated using sephadex G-100 gel filtration technique. On ultra-violet absorption of the isolated fractions was measured at 280 nm. the molecular weight of these fractions was calculated and found to be four fractions ranging :102,000,220,000,425,000 and 610.000 Daltons. Amino acid analyses showed a predominance of the following: glutamic acid ,glycine ,proline,valine,methionine and cyctine.In conclusion the information derived may be useful in the classification of Trichophyton species.