The Use of Oxidation Reaction for the Spectrophotometric Determination of Ganciclovir in Pharmaceutical Formulations


A simple and sensitive spectrophotometric method was developed for the determination of ganciclovir (GCV) in a bulk drug and in its pharmaceutical formulations. The method was based on the reduction of potassium permanganate by ganciclovir in alkaline medium to form a green colour product showing maximum absorbance at 610 nm. Beer's law is obeyed in the concentration range 2-100 µ with average recovery (accuracy) 100.24% and precision (RSD) is less than 1.0%. The molar absorptivity is 4.59x103 with LOD 0.21µ and LOQ 0.72 µ The proposed method was further applied to the determination of the drug in pharmaceutical formulations as an injection and capsule and the results are compatible with both certified values of pharmaceutical formulations and the standard addition method.