Pathogenicity of Klebsiella pneumoniae isolated from diarrheal cases among children in Kirkuk city


The isolation and identification of 61 Klebsiella pneumoniae from 433 children suffering from diarrhea have been made. The study also included determination of some virulence factors of K. pneumoniae and the results showed that all the isolates (100%) have capsule, urease, and siderophore producers. Detection of virulent isolates by Congo red binding was also studied, 14/61 (22.9%) of the isolates were binding to Congo red. Adherence test was done for 13 isolates, and all K. pneumoniae (100%) showed its ability for adherence to human buccal cavity epithelial cells. In order to study the invasion character, the mice (Balb/C) infants were sucked with the suspension of the selected virulent (according to in vitro tests) isolates of K. pneumoniae, and the histological sections were performed to study the mode of the entry of bacteria into the enterocytes, and the histological changes in the liver. Microscopical examination for histological sections showed pathological effect on two organs when compared with the control organs.