Using of High Performance Liquid Chromatography for Separation of Glutamic and Alanine Amino Acids from Myriophyllum spicatum Growing in Tigris River within Mosul City.


Two amino acids (glutamic and alanine) are separated and investigated from ethanolic extract crude of Myriophyllum spicatum growing in Tigris River within mosul city. During this study Shimadzo HPLC which included C8 (250×60 mm) colum chromatography used for this processes, with 40 mM Na2SO4 , pH 2.65 as mobile phase, again with rate flow: 1 mL / min. and detection at λ = 210 nm.