Serum interleukin-6 level using ELISA in patients with bladder cancer and having urinary tract infection


Abstract:Background: IL-6 blood levels are elevated in numerous infectious, inflammatory, and autoimmune diseases and in cancer in association with increased synthesis of other cytokines stimulated by infectionAim: This study carried out to shed light on possible role of IL-6 in bladder cancer and it's correlation with bacterial infection.Materials & Methods: Serum IL-6 levels were measured in 132 patients and 25 healthy subjects by using ELISA technique. The patients were divided into 3 groups :Group-1: Newly diagnosed bladder cancer patients, Group-2: Post-chemotherapy patients, and Group-3: Other bladder disorders rather than bladder cancer. Urinary tract infections were studied for all groups by culturing urine samples using selective culture media.Results: There was no significant elevation of mean serum IL-6 levels between different groups ,but there were higher levels in cancerous patients ,when compared with non-cancerous patients, and the mean serum IL-6 levels in bladder cancer patients in correlation to the tumor grade ,showed no significant difference, but there was significant difference of serum IL-6 levels with mean value of (5903.8765 pg/ml) in 34 Gram-negative bacterial infected patients with and without bladder cancer, and (8466.6667 pg/ml) in 3 Gram-positive bacterial infected patients with and without bladder cancer than those who were free of infection with P value< 0.001 .Conclusion: Bacterial infected patients with and without bladder cancer revealed a significant increase in the mean serum IL-6 levels by ELISA.Key words: IL-6 .serum IL-6.bladder cancer.UTI .ELISA.