In vitro and in vivo Activity of Selected Antibacterial Agents ; Alone and in Combination Against Multi Drugs Resistant Pseudomonas aeruginosa Isolated from Burns Infections

Abstract

This study included (70) swabs being collected from burned patients of both sexes ,males and females of different ages attending to burned unit at AL- Hilla Teaching Hospital in AL-Hilla city during a period of three months from October to December 2012. The results indicated that 26/70(37.01 %) of samples were Pseudomonas aeruginosa and 44/70(62.9%) revealed others types of bacteria. The susceptibility of Pseudomonas aeruginosa towards 6 antibiotics included in this study by Disk Diffusion Test showed that P. aeruginosa isolates (100%) exhited resistance for Rifampen,Gentamycin , Pipracillin , Tobramycin .While they were presently sensitive to Meropenem and Amikacin.In addition, P.aeruginosa revealed remarkable susceptibility toward natural products, Garlic Vinegar but it did not affected by Garlic Oil. According to these results the 6 antibiotics were employed for in vivo experiments , since they were used for treatment of experimentally infected animals alone and in combination .The response of experimentally infected animals in recovery from experimentally burned infection was remarkable when combined antibiotics were used for treatment in relation to the use of single antibiotics ,which indicated for synergy reaction as well and the results were in according with well diffusion method.