التأثيرات السمية لكلوريد الزئبقيك ومعالجتها بالسيلينيوم في بعض معايير الدم في ذكور الجرذان البيض

Abstract

This study was designed to investigate some side effects of mercuric chloride ,when injected intraperitonealy in healthy rats . Lethal Dose (LD50 ) was determined . it was found to be 5 mg/kg of body weight , then doses of 0.5,1.0 and 1.5 mg /kg of body weight were injected intra-peritonealy to study some blood parameters for a period of 45 days . Sixty eight male rats weighed 200 - 300 grams & aged (12) weeks were used in this study . twenty rats were divided randomly to determine the Lethal Dose (LD50) , and 48 rats were divided randomly into four groups (12 rats for each group) ,the first animal group were injected subcutaneously with normal physiological saline ,while 2,3,4 groups were injected with mercuric chloride at doses of (0.5,1.0,1.5 ) mg /kg of body. The results showed that there was a significant decrease in Packed Cells Volume (P.C.V) , Red Blood Cells ( R.B.Cs ) & no significant differences in White Blood Cells ( W.B.Cs ) , also showed a decrease of liver enzymes ( ALT,AST,ALP ) & total protein in serum . Selenium at the dose of ( 0.5 ) mg/kg of body weight for (30 days ) to decrease the ef-fects of mercuric chloride was injected subcutaneously in the rats that were already treated with mercuric chloride . Significant decreases in Packed Cells Volume (P.C.V) & Red Blood Cells count (R.B.Cs) were found & no significant differences in White Blood Cells (W.B.Cs) and in ( ALT,AST,ALP ) were noticed & a decrease in total serum protein was observed .