Determination Effect of Common Used Insecticide as Dichlorvous, Diazinon and Cypermethrin in vitro in the Camel

Abstract

Using modified electrometric method to validate the measurement of plasma and erythrocyte cholinesterase activities camel. At several temperature degrees 37.5 lead to given an optimum enzymatic reaction. The enzyme activity was expressed as ΔPH / 30 min. The Confidence limit in the plasma were 0.071 respectively and those of the erythrocytes were 0.148 respectively. The organophosphorus insecticides diazinon at concentration (0.25- 2 µM) in the reaction mixture significantly inhibited plasma (31~43%) and erythrocyte (52~67%) and dichlorvous at concentration (0.25 - 2 µM) in the reaction mixture significantly inhibited plasma (29~46%) and erythrocyte (55~68%) cholinesterase in vitro in a concentration-dependent. The insecticides cypermethrin at concentration (0.25 - 2 µM) in the reaction mixture significantly inhibited plasma lactic dehydrogenase by (19~22%). This experiments show activates of enzymes that affected by common insecticides used in Iraq.