Detection of GAD¬65 Antibodies in Newly Onset Type 1 Diabetic Children


Summary:Back ground: Glutamic acid decarboxylase (GAD) has been defined as a major target antigen in type 1 diabetes mellitus (T1DM).Aim of the study: Assessment of GAD¬65 autoantibodies in the serum of T1DM children at onset of the disease.Patients & Methods: Serum GAD¬65 autoantibodies has been estimated in 60 sera samples of newly diagnosed T1DM children (diagnosed less than 5 months) in comparison with 50 healthy control children using IRMA method.Results & conclusion: A higher significant proportion of the patients were positive to GAD65 autoantibodies (50%) in comparisons with healthy controls (4.76%) in age group ≤10 years old and (6.90%) in > 10years old (p<0.0001), whereas a significant proportion of girls tested positive for GADA were in age group >10years old (p<0.05). We concluded that GADA are an excellent diagnostic marker for T1DM.Key wards: GAD autoantibodies, T1DM.