Effect of different concentrations of different extracts of Ginger (Aqueous and Alcoholic) in the inhibition of Salmonella typhimurium isolated from some cases of diarrhea in calves in vitro

Abstract

This study was carried out to investigate the effect of both extracts of ginger (aqueous and alcoholic) in three different concentrations in the inhibition of Salmonella typhimurium isolated from some cases of diarrhea in calves in vitro. To achieve this aim this study included two main aspects as follow: (1. Isolation and identification of Salmonella typhimurium from dirrhea infection of calves. For this purpose, 5 samples of faeces from infected calves were collected from animal field of Agriculture College, University of Baghdad. These faecal samples were inoculated on aselected media including solid salmonella-shigella medium, xylose lysine deoxy cholate XLD medium and solid MacConkey medium then incubated at 37c for 24 h. According to the growth features and the microscopical examination of gram stained cells and some biochemical tests only one isolate of Salmonella typhimurium from the diarrhea cases of calves was isolated. (2. The inhibitory effects of both extracts of ginger (aqueous and alcoholic) in three different concentrations for each (0.25, 0.5 and 1.0 g/ml) against Salmonella typhimurium bacteria. Results showed that the three different concentratins of alcoholic ginger extract have strong inhibitory effect on growth of Salmonella typhimurium bacteria the dimeter of inhibited zone was (4-10-12) mm respectively, while the three concentrations of aqueous extract didn’t showed any inhibitory effect against this bacteria