Biological study for effect's of Nigella sativa extract on some character's local E. coli

Abstract

This study was carried out to know the effect of the activity of antimicrobial of the watery and alcoholic extract that concerns Nigella sativa besides the interaction of these two extracts with different five antibiotics against nine local isolate from E.coli which are isolated and diagnosed and taken from Ramadi Hospital for Maternity and children. From another aspect, the study aims at knowing the effect of the existence of the extract of Nigella sativa with different concentrations within the culture media that regards one of the isolates and the reflection of that on the response of the isolate against some antibiotics. The saturated disc diffusion was used to know the effect of the antibiotics and the watery and alcoholic extract and their interaction by testing the least significant difference. There is a significant difference in the response of the isolates to the effect of these two extracts and their interaction with some antibiotics since the local isolate No.4 is characterized by its response against the interaction of alcoholic extract of Nigella sativa with the antibiotic Vancomycin (VA) to make the inhibition zone 19.5 mm and against the watery extract to make the inhibition zone 17 mm taken into account that there is no response when there is only the antibiotic