EFFECT OF OMEGA3+VITAMIN E ON EXPERIMENTAL INFECTION OF LABORATORY MICE WITH PSEUDOMONAS AERUGINOSA AND KLEBSIELLA PNEUMONIAE

Abstract

In present study, 40 adult male mice were used and divided into 5 equal groups of8 mice each. The first group was the control group, in which the mice were fed withstandard ration along the period of experiment; the second and third groups were fedon fats free ration for 14 days while the fourth and fifth groups were fed onOmega3+Vitamin E supplemented ration for 14 days too. The test organisms weresuspended in phosphate buffer solution (PBS) and 10-folds serial dilutions were donefor (CFU) count. Then, the animals of the second and fourth groups were injectedintramuscularly with 22×105 (CFU) of Pseudomonas aeruginosa bacteria, while theanimals of the third and fifth groups were injected intramuscularly with 18×106 (CFU)of Klebsiella pneumoniae bacteria. After 24 hours all mice of the third group and 3mice of the fifth group died; their thigh muscles, livers, lungs, spleens and stomachswere taken, weighed and homogenized with phosphate buffer solution (PBS) to countthe (CFU) in these organs. 2 days later, the remainder 5 mice of the fifth group wereeuthanized and the previous processes were done. For the second and fourth groups,after 48 hours of the bacterial injection all mice of the second group and 4 mice of thefourth group died, the previous processes were done for the bacterial count. 2 dayslater the remainder 4 mice of the fourth group were euthanized for the bacterial countpurpose. The results revealed that the use of Omega3+Vitamin E as a supplements toration increased the survival of mice and the organs bacterial count of theOmega3+Vitamin E fed mice decreased significantly as compared to those of the fatsfree fed animals at (P≤0.05).