In vitro effects of terpinoid compounds of Euphorbia helioscopia L. extracts on rats epididymal sperm activity rats


Background:Many plants have medical importance due to the presence an active compounds in plants extraction acting as a drugs for treatment of several diseases in human and animals. Euphrobia helioscopia extract was used to treat diabetes, removal the warts of the skin, stimulate the nervous system and also used against constipation.Objective:Study the effect of terpinoid extract of E.helioscopia on rat epididymal sperm activity and fertility efficacy of male rats.Methods:This study was conducted at Biol. Dept., College of Science/ University of Babylon and Medical College, Mahidol University- Thailand. The study was designed to purify the active compounds of the Terpinoid of Euphorbia helioscopia extract by Nuclear Magnetic Resonance (NMR), and studying their effects on sperm motility and grade activity of the epididymal sperms of the rats. Six main active compounds were isolated from the terpinoid extract of E. helioscopia due to the differences in color, weight and nature by using NMR. The main terpinoid compounds were named A,B,C,D,E and F.Two concentration (0.5 mg/ml and 1 mg/ml) of each active compound of £. helioscopia extract were added to sperm suspension in vitro and incubated for 30 minutes , then sperm motility and grade activity were assessed and the results were compared to those values before adding the active compounds (control).Results:The results showed that 0.5 mg/ml of C and D active terpinoid compounds caused significant (p<0.05) decrease in sperm motility compared to before adding the extract, while the same concentration of B,C,D,E and F active terpinoids compound caused a significant (p<0.05) decrease in grade activity. The addition of 1 mg/ml of terpinoid active compounds (C,D and F) caused a significant (p<0.05) decrease in sperm motility, while the grade activity significantly (p<0.05) decreased by adding B,C,D,E and F active terpinoid compounds compared to control. The sub - compounds (C1,C2,D1,D2,D3 and D4) had a powerful decreasing effect on the sperm motility and grade activity compared to control and other active compounds.Conclusion:It was concluded that terpinoid compounds of E. helioscopia extract have adverse effects on rat epididymal sperm activity.