Development of Indirect Spctrophotometric Method for the Determination of Clonazepam in pharmaceutical preparation Using Resorcinol


This paper describes the development of a spectrophotometric method for the determination of Clonrazepam after diazotization of its reduced product and coupling with Resorcinol in basic medium , to form an intense yellow – colored , water – soluble and stable azo-dye which shows a maximum absorption at 341 nm. Beer’s law is obeyed over the concentration range 1.2 to 20 ppm with a molar absorptivity 4.5147×104 and Sandell’s sensitivity of 6.993×10-2μ The relative error is ranged from -0.017 to 2.74 % and relative standard deviation from ±0.001 to ±0.2636 % , depending on the concentration level. The method has been applied successfully to the determination of Clonazepam in pharmaceutical preparation .