The Effect of H2O2 and Some Antioxidants on Human Sperm Parameters in vitro


This study was performed to determine the effect of H2O2 addition to the semen specimens to stimulate the formation of free radicals, and treated with antioxidants. This study involved ten semen specimens collected from fertile men. Seminal fluid analysis was performed to estimation sperm parameters and Malondialdehyde (MDA) concentration. Also seminal fluid analysis and MDA level was determination after adding of H2O2 and then antioxidants (VE, VC, VE+VC, glutathione and seminal plasma).The results revealed a significant decrease (p<0.05) in sperm motility percent, grade activity and sperm viability percent, and significant increase (p<0.05) in MDA concentration by addition 0.1% of H2O2 to semen specimens compared to those values with normal saline. The addition of antioxidants (0.6mg/ml VE, 0.4mg/ml Vc + 0.6mg/ml VE, 0.4rng/ml glutathione and seminal plasma) to the semen specimens contain 0.1% H2O2 caused significant increase(p<0.05) in sperm motility percent, grade activity and sperm viability percent , and significant decrease (p<0.05) in MDA level compared to those values in semen samples contain 0.1% H202 alone. While VC caused insignificant differences (p>0.05) in all sperm parameters and MDA concentration.