Flow-Injection and Batch Spectrophotometric Methods for Determination of Metoclopramide .HCl in Pharmaceutical Formulations


Background: This study describes a batch and flow-injection spectrophotometric methods for the determination of Metoclopramide.HCI in aqueous solution and in pharmaceuticalpreparations.The method is based on the reaction of Metoclopramide.HCI with ferric nitrate to produce Fe(II) ion which upon further reaction with potassium hexacyanoferrate in acidic medium forms a Prussian blue colored product that has a maximum absorption at 770 nm. The proposed method was successfully applied to determine Metoclopramide.HCI in the range of 0.2-6 μg.ml-1 for the batch spectrophotometric method and 2-80 μg.ml-1 for flow-injection (FI) spectrophotometric method with correlation coefficients of 0.9997 and 0.9999, deletion limits of 0.114 μg.ml-1and 0.700 μg.ml-1, and relative standard deviations of 0.46% and 0.41% respectively. Both procedures were applied to analyze the assay of Metoclopramide.HCI in pharmaceutical preparation with recoveries of 99.46% and 99.85% respectively.Objectives: The aim of this study is to investigate A simple accurate and sensitive FIA system to determine Metoclopramid.HCL in pharmaceutical preparations.