توزيع انواع الجنس Citrobacter ما بين العينات السريرية والعينات البيئية للمستشفى التعليمي في جامعة النهرين

Abstract

Four hundred and thirty eight specimens were collected from educational hospital in Al-Nahrain University, including 250 clinical specimens from patients such as (stool, rectal swabs, urine, blood, sputum, cerebrospinal fluid and wounds ,ear ,nasal and throat swabs) and 188 environmental specimens from patient halls and operation rooms in the hospital . The control specimens included 50 stool specimens from healthy adults and 20 stool specimens and rectal swabs from healthy neonates.Eight isolates of the genus Citrobacter were obtained from both clinical and environmental specimens and one isolate were obtained from control specimens. These isolates were identified according to morphological and biochemical tests, furthermore, the identification was confirmed using the Api 20 E systems.Three species belonging to the genus Citrobacter have been obtained, these are C. freundii, C. farmeri and C.braakii, the former was the most frequent species with isolation percentage about 75% from clinical isolates and 50% from environmental isolates.The sensitivity of C. freundii against antibiotics was tested and the results showed that naldixic acid, nitrofurantoin and neomycin were found to be the most effective against local isolates of C. freundii with percentage of sensitivity 100% and their sensitivity to gentamicin was about 60%, while all isolates were resistant to -lactams, tetracycline and chloremphenicol.