Effect of Abiotic Stimulaterson Ajmalicine production from leaves induced callus of Catharanthusroseus L. G. Don in vitro


Abstract: An experiment on the effect of Stimulates L-Tryptophan ,salicylic acid and NaCl on leave induced callus on Ajmalicine production of Catharanthusroseus was conducted from February 2011 to May 2012. Callus induction was achieved by culturing true leaves of the plant on MS supplemented with 0.5 mg /L 2,4-D and 1mg / L Kin. The best medium to maintains callus was MS medium modified with 0.5 mg /L 2,4-D and 1.5 mg / L Kin. When different levels are added to MS medium for each L-Tryptophan 0,200,300,400 mg /L andsalicylic acid 0, 0.5,1, 1.5 mg /L and NaCl 0,1,2,3,4 g /L in split experimental and the medium supplement with 30 g/ L sucrose as control. The results showed medium supplement with 200 mgL of L-Tryptophan and 1 mg L of salicylic acid gave the highest quantity of Ajmalicine it was 26.18, 75.02 µg/100 fresh weight of callus respectively. The concentration 3 g/ L0f NaCl gave the addition of Ajmalicine production equal to11.27 µg/100 fresh weight of callus while the concentrations of Ajmalicine derived from the leaves of the plants discarded in the Pot were lowest than the concentrations produced by the callus grown in vitro. The concentration of Ajmalicine was 0.047µg/100 fresh weights of leaves.