Assessment of Genetic Effects of Bacterial Cells After Exposure to Mobile Phone Radiation Using RAPD Technique


Abstract: In this study the random amplified polymorphic DNA (RAPD) technique was used to evaluate the genetic effects of the DNA of Escherichia coli and Staphylococcus aureus bacteria after exposed to various durations (15, 30, and 45 min) of radiofrequency (RF) radiation used in cellular phone communications at a frequency of 900 MHz compared with control (unexposed). Genomic DNA were obtained from all bacterial cells, then amplified individually by RAPD technique with fourteen different 10-mer arbitrary primers. Change in the genome revealed by RAPD technique included variation in band intensity; loss of normal bands and appearance of new bands compared with control. The results display that exposition to various durations of mobile phone radiation induced great changes in RAPD profiles of genomic DNA of bacterial cells compared with control and genomic DNA of E. coli bacteria was more affected than S. aureus. In addition, the results demonstrated that RAPD technique is useful for detection of alterations in the DNA produced by mobile phone radiation.