Pulsed laser ablation synthesized silver nanoparticles induce apoptosis in human glioblastoma cell line and possess minimal defect in mice brains


Abstract: In this research we have assessed the antiproliferative activity of silver nanoparticles (SNP) synthesized by pulsed laser ablation in liquid (PLAL) method. Shape and particle size distribution of the synthesized nanoparticles was characterized with atomic force microscope (AFM) surface plasmon resonance was determined with UV-Vis spectroscopy. A silver nanoparticles concentration in the prepared liquid was determined by atomic absorption spectroscopy. Cytotoxic activity of three different concentrations 15, 30 and 60 µg/ml of SNP against human glioblastoma cell line (AMGM) was assessed with MTT assay. Possibilities of apoptosis induction were inspected using acridine orange/ ethidium bromide staining and DNA fragmentation assay. Toxicity of the synthesized SNP on normal brain cells was assessed in vivo using BALB/c mice. These results indicated that SNP was able to inhibit AMGM growth due to induction of apoptosis and their damage effect toward normal brain cells were at minimal according to histotoxopathological inspection.