The effect of lead acetate and magnetic water on total protein and enzymes on Broiler

Abstract

The aim of the study was designated to follow up the effect of lead acetate and magnetic water on the amount of total protein and electrophoresis protein as well as the activity of some enzymes (ALP, AST, ALT) using spectrophotometric and The electrophoresis method was also used to follow up the effects on the Esterase bands. 108 broiler one day old were divided randomly in to 3 treatment with a control group. Each treatment subdivided to 3 replicates, the first treatment (T1) was received lead acetate in a concentration of 400 mg/ l drinking water, the second (T2) group was treated with magnetized water strongly treated with 3000 Gaus and the third (T3) group was received lead acetate in concentration 400 mg l to the magnetized water. The results showed that there was significant decreased in total protein levels in T1 and T3 and increased in total protein levels in T2. A significant increased in the level of AST enzyme in all treatment and increased in the levels of ALP and ALT in T1 and T3 and observed changes in number and density of protein and Esterase bundles on Poly acrylamide gel electrophoresis. It was concluded from this study that lead acetate has an effect on the total protein levels and some enzymes and the use of magnetic water reduce the effect of lead acetate