Detection of bovine corona virus in some governorate of Iraq

Abstract

In the present study we used Reverse transcription polymer chain reaction (RT-PCR) assay for detecting of bovine corona virus ,We evaluated presence of bovine corona virus ( BCoV) in diarrheic fecal samples in age (1 – 30)day . (152) faecal samples from diarrheic calves were collected from four Iraqi governorates (Al-Qadisyia, Babylon ,Wassit and Najaf) .10 (6.57%) out of 152 were positive to bovine corona virus . This study is the first detection of bovine corona antigen in Iraq .The results suggest that RT-PCR is more sensitive than other method to detect BCoV, especially in subclinical cases and Because these animals shed a low amount of virus in faeces and more animal show clinically normal we need to apply sensitive techniques, such as RT-PCR .