The effect of Alcoholic plant (tribulus terrestris) extract on the bacteria isolated from genital tract of infertile cows in vitro


Out of Two hundred swabs collected from genital tract of infertile cows during the period between7/1/2012 to 30/6-2012. Cultured on Blood agar, Nutrient agar, MacConkey agar and Mannitol salt agar. 195 swab represented (97.5%) give positive result and 5 swab (2.5%) give negative result. from the positive culture 78 isolated (39%) mixed bacterial infection. All bacteria isolates were identified by using different biochemical tests and API-20E, the single bacterial isolation in this study were Esherichia coli 41.8%, Staphylococcus aureus36.8%, Pseudomonas aeruginosa17%, Citrobacter braakii1.7%, Providencia rettergi1.7% and Shewanella putrefaciens 0.9%. In mixed bacterial isolation, the highest percentage was 53.8% Staphylococcus + Esherishia coli aureus and the lowest percentage was 1.3% Esherishia coli + Citrobacter braakii + Pseudomonas aeruginosa. Antibiotic susceptibility test were carried out on bacterial isolates using 10 single antibiotic discs of commonly used drug, the test showed that most bacterial isolated resist for most antibiotics (Amikacin, Amoxicillin, Nalidix acid, (Amoxicillin and Clavulanic acid), Cefixime, Ceftriaxone, Tetracycline, Nitrofurantoin, Norfloxacin and Gentamycin) on the other hand observed sensitive for alcoholic plant tribulus terrestris extract (fruit) in different concentrations