SEROLOGICAL STUDY FOR SOME CHICKEN ALLERGENS IN ALLERGIC PATIENTS

Abstract

An allergic extracts from chicken feather and dropping were prepared with extraction, followed by purification using dialysis.Total and specific IgE ELISA was performed on a total of 190 serum samples collected from allergic and healthy individuales in center of asthma and allergic diseases in Basrha city during September 2011.ELISA test based on total IgE results revealed that the higher rate of allergic patients (47.37%) had allergy questionable while (35.26%) of them had allergy very probable and (17.37%) of them infected with allergy not probable. According to the relationship between the symptoms of allergy and total IgE based ELISA results, the higher rate of allergy very probable 95.5% was observed in symptomatic patients.According to the results of ELISA based on specific IgE the overall rate of chicken allergens in studied patients 94.7%. Chicken dropping allergens showed the higher overall 94.7% of distribution followed by the rate of feather allergens 73.68%. Depending on the sex, age (first age group range from>15-45 and second age group range from <45-75) and type of sensitivity to all tested allergens, males and the patients of second age group showed higher rate of sensitivity ( 80.35% and 80% respectively ), while in patients who were sensitive to single allergen (Dropping) the higher rate of sensitivity was observed in females and first age groups patients (23.88% and 15% respectively).The estimation of IgE seropositivity in symptomatic and asymptomatic individuals resulted the symptomatic patients showed the higher rate of seropositivity ( 95.8% )against chicken dropping allergens, while the higher rate of seropositivity (75%) was observed against chicken feather allergens in asymptomatic patients.According to the relationship between total and specific IgE based ELISA results, the patients with allergy very probable showed the higher rate of seropositivity for both dropping and feather allergens (98.5% and 76.1% respectively).The positive feather and dropping based ELISA results (mean± SD) of the allergic patients sera depending of sex and age revealed different mean ± SD value of optical density in concern to sex and age of allergic patients

Keywords

Allergic, Serum, IgE