Genetic distribution of the southern cowpea bruchid Callosobruchus maculatus (F.) (Coleoptera: Bruchidae) in three different locations in Iraq


Due to little information’s about the molecular genetics of the southern cowpea insect Callosobruchus maculatus Fab. (Coleoptera: Bruchidae) in Iraq , therefore this investigation was done to study the genetic differences using of PCR technique )Polymerase Chain Reaction) and amplifying nuclear gene (28Sr DNA) with specific primers for three community populations of this insect taken from three cities in Iraq Kirkuk, Erbil and Tikrit from infested local cowpea seeds Vigna unguiculata L. (Leguminaceae or Fabaceae). Results revealed the similarity in phenotypes of C. maculatus populations and appearances biotypes difference from each other in nucleotide sequences ,and the genetic distance among samples ranged between ( 0 - 0.26).