The Study of some Plant Extracts Effect on Free Radicals Removal from DNA in Vetro


The removal efficiency of Several plant extracts of Nagilla sativa linn, Allium sativum sativum and Allium sativum ophioscordon of free radicals from pure DNA molecule at concentration 5 µgm / ml have been studied when we added 1×10-5 M of H2O2 with plant extracts mixture by measuring absorbency at 260 nanometer . Breaking index for nitrogen base pair was calculated. The plant extracts showed high ability in free radicals scavenge when used in concentrations (0.1, 1, 10, 100) µgm / ml which effect in DNA molecule. Also the DNase activity from different plant extracts was measured in DNase agar. Alcoholic mixture activity of three plants in (1،10،100) µgm/ml concentration was studied by monitoring the protection effect on pBR322 plasmid DNA from Escherichia coli which was protect t pBR322 plasmid he plasmid DNA from breaking by H2O2.