Immunohistochemical study of CD15 expression in CD30+ classical Hodgkin's lymphoma


Background: Hodgkin lymphoma (HL) is a histologically defined B-cell neoplasm that includes two distinct types of disease, classical and nodular lymphocyte-predominant.Aim of the study: To evaluate the immunohistochemical expression of CD15 in CD30+ classical Hodgkin’s lymphoma.Patients and Methods: This retrospective study included revision of 52 lymph nodes specimens from patients newly diagnosed with HL from January 2005 to September 2010. These samples were collected from Al-Yarmouk Teaching Hospital, Baghdad Medical City and Al-Atheer private laboratory. Only well-preserved samples that stood a retrospective confirmatory morphological revision and revealed IHC CD30 stain {from DAKO} positive reactivity were included in this study.All biopsies were formalin-fixed and paraffin embedded. A 4 micron sections obtained and stained with hematoxylin and eosin and CD15 monoclonal antibody {from DAKO} according to the kit-included instructions.Results: This study revealed that the CD15 positive classical HL are 24 (75%), 10 (100%), 6 (100%) and 0 (0%) for MC, NS, LD and LR respectively.Conclusions: All cases of cHD should undergo immunophenotypic analysis for CD15 in addition to CD30 antigen.