The relationship between the site of infection and virulence of Pseudomonas aeruginosa experimental infection in mice


The aims of this study were to investigate the virulence of Pseudomonas aeruginosa depending on infection site from otitis media in mice and compared with inter peritoneal infection. Suspension of bacteria was prepared in three doses(103, 106and108 ) CFU/ml live attenuated P. aeruginosa from otitis media and peritoneum infection of mice were tested in sixty healthy mice (White PALB/C), which randomly divided in two groups, first group isolated from otorrhea of otitis media , second group isolated from interperitonium infection. Both groups were subdivided in to 5 groups, each subgroup have 6 mice, were infected orally, subcutaneous , urinary tract and eye infection with 0.5 ml of bacterial suspension. While the 5th group was control negative. The present study found that the adhesion of P. aeruginosa isolated from otitis media isolates to mice epithelial cell was significantly increased compared with the isolates from peritoneal infection, the resistance to infection showed multiple resistances of otitis media isolates to antibiotics more than peritoneum isolates.