ISOLATION SOME ACTIVE MATERIALS IN THE IRAQI FRUITS AND SEEDS OF CITRULLUS COLOCYNTHIS L. AND STUDY OF THEIR EFFECTS ON AQUEOUS AND ALCOHOLIC EXTRACTS OF SOME PATHOLOGICAL BACTRIA AND CANCER CELLS TYPE L20B

Abstract

The aim of this research is to detect and identify the functional chemical materials in the fruits and seeds of citrulluscolocynthisL..The results show that the fruits contain Tannins , phlobatannins , Saponins , flavonoids , glycosides , terepens , phenols , resins , carbohydrates , alkaloids , and iridoiglycosids . while the seeds contain tannins , saponins , flavonoids , terepens , phenols , resin , amino acids , carbohydrates , alkaloids , and iridoidglycosides .The percentage of isolated saponins , tannins , and volatile oils in fruits 10.92% , 17.6% , and 2.04% whereas their percentage in seeds are 3.77% , 7.39% , and 18.7% respectively .The concentrations of isolated materials , aqueous extracts and alcoholic extracts (1 , 5 , 12 , 25 , 50 mg/ml )have been studied as a biological activities against E.coli and staph.aureus the results show the highest inhibition diameter is 18mm against E,coli using 12mg /ml while against staph.aureus is 20mm using 25mg/ml concentration .The aqueous and alcoholic extracts of fruits and seeds gave a good inhibition towards the growth of L20B cell line and the highest growth inhibition is 67.7% .