استخلاص وتنقية البورين من بكتريا Serratia marcescens وتطبيقه على خطين من الخلايا

Abstract

The bacteria Serratia marcescens conseder one from pathogenic humen bacteria have several virulence factors, Was isolated one virulans factor outer membrane protein (Porins) was purified and characterization using standard methods applied by several research. Purification way essentially depended on salting extraction followed by gel filtration using Sepharose -6-B chromatography. Molecular weight of porins was estimated using sodium dodecyl sulphate- polyacrylamide gel electrophoresis SDS – PAGE and it appears to be 36 and 38 kDa , respectively . The final prepration contained porins in a final concentration of 2.91 mg / ml. The final part of study involved the application of the extracted porins using defferent concentrations (0,50,100,200 µg/ml) for the selected two cell lines macrophages and lymphocyte cells, and some results showed that some concentration of porin effective in limited ratio on these cells.So (200) concentration That will give a highly effective on macrophage cells , While (100,200) concentrations have given highly effective on lymphocyte cells .