Some Immunological Evaluations of Propolis in Albino Male Mice


Propolis is a complex resinous bee product that has a wide range of biological activities. In thepresent investigation, two oral doses (10 and 20 mg/kg/day) of propolis (ethanol extract) wereevaluated immunologically in albino male mice (80 animals) through three types of experiments. Inthe first, the propolis was tested alone, while in the second and third experiments, propolis was givenbefore and after the immune suppressive drug MMC (pre- and posttreatments, respectively). The three experiments were paralleled with three negative controls, inwhich the propolis was replaced with distilled water. In the first experiment, the dose 10 mg/kg ofpropolis enhanced the parameters investigated, and a significant increase was observed in the totalcount of leucocytes (10.7 vs. 7.8 x 103 cells/, lymphocytes (7.0 vs. 5.3 x 103cells/, neutrophils (2.9 vs. 2.1 x 103 cells/, monocytes(0.5 vs. 0.3 x 103 cells/ and eosinophils (0.3 vs. 0.1 x 103 cells/ blood), PI (15.2vs. 10.8%), PFC (72 vs. 38%), AR (0.84 vs. 0.57 mm) and DTH (0.68 vs. 0.40) as compared tonegative controls. Much more enhancements were observed in the dose 20 mg/kg. In the second andthird experiments, a similar picture was drawn in the interaction of propolis (pre- and posttreatments)with MMC, in which the propolis extract was able to modulate the immune suppressiveeffect of MMC, and this was dependent on the type of treatment and dose, and again, the dose 20mg/kg was more effective in this respect.Key words and Abbreviations: Arthus reaction (AR), Delayed type hypersensitivity reaction (DTH),Mitomycin C (MMC), Phagocytic index (PI),Plaque forming cells (PFC), Propolis and Sheep red blood cells (SRBC).