Thirty serum samples were collected from patients with chronic hepatitis B who were admitted to Hepatology and Gastroenterology Teaching Hospital in Baghdad during period from beginning of February / 2008 to the end of February / 2009, also serum samples were collected from 30 healthy HBs Ag carrier was discovered accidentally through attending blood bank for donation of blood and a total of 30 apparently healthy blood donors were included in the study served as a control group. The concentrations of some cytokines Pg/ml (IFN-γ, GM-CSF, IL-1α, IL-6, IL-8 and IL- 2 receptor) have been estimated in the sera of the studied groups and the results showed that mean levels of gamma-interferon, interleukin-1 alpha, interleukin-8 and interleukins-6 in sera of carriers group were (109.6, 251.6, 232.9 and 166.8 respectively) more than the level of these cytokines in sera of chronic HBV patients (75.1, 94.7, 159.6 and 110.9 respectively) and healthy control (48.4, 10.1, 11.3 and 15.4 respectively), while the mean level of GM-CSF was elevated in sera of CHB patients (110.8) than both of healthy control and carrier group (17.3 and 41.2 respectively).Finally the interleukin-2 receptor was represented with similar level in sera of chronic patients and carrier group ( 165.6 and 167.9 respectively) with non significant difference, whereas all other statistical comparisons among the three main groups showed highly significant difference.