LECTIN EXTRACTION AND PARTIAL PURIFICATION FROM BACTERIA KLEBSIELLA PNEUMONIA ISOLATED FROM PATIENT WITH URINARY TRACT INFECTION AND SOME APPLICATION

Abstract

The study involves identifying the strains of Klebsiella pneumonae which isolated from patients with urinary tract infections, depending on the morphology, culturing and biochemical characteristics of the colonies, and extraction of mannose specific Lectin from the bacterial pili by breaking them using a set of super sonic waves in different periods of time. The sonication results showed that the higher agglutination activity (512) of the bacterial extract was recored in 4 min sonication. The extract and purified mannos specific Lectin have shown a sensitive agglutination activity against human blood groups (A,B and O) and animals red blood cells ( Geniua pig, Sheep, Cow, Chiken, Rabbit, Rat, Goat and Mouse).Also lectin showed to have a highest agglutination activity against some bacteria E. coli, Aero. hydrophila, P. aeruginosa, but not against Staph. aereus and Proteouas spp. Bacteria.