IN VITRO ADVENTITIOUS BUD FORMATION FROM THE CALLUS INDUCED ON IMMATURE INFLORESCENCE OF DATE PALM (Phoenix dactylifera L.)

Abstract

This research was conducted to study the possibility of using different parts of immature inflorescence of date palm cvs. Barhi and Maktom at different developmental stages as explants in order to induce callus formation, adventitious buds formation and elongation of these buds. Results showed that the best percentage of the in vitro response to callus formation reached 40 and 70% for Barhi and Maktom respectively from spike segments with 0.5cm excised from 8-10cm spath and cultured on modified MS medium supplemented with 100µM 2,4-D with 10.0µM 2ip. The highest callus fresh weight (286.3mg) was achieved on the same above medium. Results also indicated that 80% of the cultures gave 11.2 and 24.8 buds for Barhi and Maktom respectively. This was achieved when callus was transferred to a liquid shaking medium supplemented with 10.0µM 2ip and 5.0µM NAA after 43 days for Maktom cv. Shoot elongation occurred after the addition of various concentrations of adenine sulfate. The highest shoot length (4.52)cm occurred in Maktom cv. with 100mg/L of adenine sulfate and 4.41cm in Barhi cv. with 80mg/L. Accordingly, it is possible to use immature inflorescences excised from adult date palm trees for the micropropagation of Barhi and Maktom cvs.