The role of high dose of Pomegranate on P53 expression in healthy and Dacarbazin’s treated experimental male rats

Abstract

The possible action of high dose of pomegranate (Punica granatum L.) powder extract was evaluated innormal healthy and dacarbazine-induced toxicity in albino rats. Twenty-eight male albino rats were usedin this study. The animals were divided into three different groups in addition to control group. The first grouptreated with intravenous injection of (1mg/kg b.w.) dacarbazine. he rats of the second group supplemented withpomegranate juice with concentration (1000mg/l) in drinking water, while the third group was the combinationof the two treatments. Samples of blood were collected after a weeks of treating, and the serum was obtainedin the laboratory for the evaluation of P53 protein within the serum. The results showed a significant reductionof P53 level in dacarbazine treated rats comparing with control. Whereas, pomegranate juice treated rats hadsignificant increasing of P53 level as compared to control. The level of the combination treatment also wassignificantly more than control and dacarbazine treatment, while it was significantly lesser than pomegranatejuice treated rats. These finding suggests that dacarbazine cytotoxicity is not P53 dependent and high dose ofpomegranate has role in P53 protein expression induction.