RELATIONSHIP BETWEEN THE EXPRESSION OF CD34, CD123 AND MYELOPEROXIDASE MARKERS BY FLOW CYTOMETRY AND RESPONSE TO INDUCTION THERAPY IN ACUTE MYELOID LEUKEMIA

Abstract

Background:Different immunophenotypic markers were found to be related to the prognosis of acute myeloid leukemia. Among them are CD34, CD123 and myeloperoxidase.Objective:To evaluate the relationship between the expression of CD34, CD123 and myeloperoxidase markers by flow cytometry; and the initial response to induction therapy in acute myeloid leukemia patients.Method:A cohort of forty one patients with newly diagnosed de novo acute myeloid leukemia were prospectively tested for the expression of CD34, CD123 and myeloperoxidase using multicolor flow cytometry and re-evaluated for the response to a 7+3 induction therapy regimen.Results:It was found that 64.29% of CD123- patients achieved complete remission while 70.37% of CD123+ patients not (P = 0.035). For CD34, 55.56% of CD34- patients achieved complete remission while 63.64% of CD34+ cases not. The induction failure in CD34+M3 cases was 100% (P = 0.045). Regarding myeloperoxidase, 61.54% of patients who had >20% myeloperoxidase expression achieved complete remission while 70.37% (myeloperoxidase expression in <20% of cells) failed to achieve complete remission (P = 0.05).Conclusion:Expression of CD34 and CD123 and weak expression of myeloperoxidase (<20% of blast cells) are associated with poor response to induction therapy in acute myeloid leukemia patients.Keywords:Flow cytometry, CD34, CD123, myeloperoxidase, acute myeloid leukemia