THE GENETIC DIVERSITY BETWEEN MAIZE INBRED USING RAPD

Abstract

This study was carried at the Agricultural Research Laboratories during 2013. The objective was to investigate genetic diversity among 10 maize inbred lines. The random amplified polymorphic DNA (RAPD) based on PCR with 12 primers. RAPD DNA markers were used to evaluate trends in genetic diversity among inbreds. All of the RAPD primers used for initial screening were found to be polymorphic. A total of 108 DNA bands were generated by 11 random primers with an average of 10.7 per primer. The number 0f amplified bands produced per primer ranged from 5 for OPAV-03 primer to 17 for OPAW-10 with molecular length ranged from 160 bp to 1800 bp. The total number of polymorphic bands and % were 74 and 62.7%, respectively. Maximum level of polymorphism was 80% observed with the primer OPAK -15, while primer OPAW-11 showed the lowest percentage. Based on the bivariate (1-0) data and genetic similarity with the use of UPGMA cluster method, the dendrogram separated the studied populations into A and B. Cluster compared among lines into dendrogram, showed BK104 highest genetic diversity with other inbreds.The high genetic diversity was found between BK104 and BK164. The highest genetic similarities 0.839 was observed between BK164 and BK147. While the highest genetic diversity was found between inbred line BK104, BK128 and BK104, BK164 (0.377, 0.396), respectively. The results indicated that DNA molecular markers were highly efficient in detecting the purity and genetic relationship among maize inbred lines.