Immunophenotyping of Bone Marrow Biopsies in 26 Patients with Non-Hodgkin's Lymphoma, Using Anti-CD3, CD8, CD19 and CD20 Monoclonal Antibodies


ABSTRACT:BACKGROUND:Immunophenotyping with monoclonal antibodies (MoAbs) directed against lymphoid-associated antigens, immunohistochemical staining on paraffin-embedded BM biopsy material, and molecular studies of Ig genes/T-cell receptor genes or lymphoma-associated gene translocations should be used in the global approach to the patient with malignant lymphoma.OBJECTIVE:1.To determine the subtypes of non-Hodgkin lymphomas (B- or T cell) in the bone marrow using anti-CD3, CD8 monoclonal antibodies for T-cell and anti-CD19 and CD20 for B-cells. 2.Correlation of the subtypes of NHL (B- or T cell) with the morphology and pattern of bone marrow infiltration. PATIENTS, MATERIALS AND METHODS:A retrospective study, done in Al-Kadhymia teaching hospital during the period from 1/10/2010 to1/2/2011.The study consisted 26 adult patients, who were diagnosed as Non-Hodgkin lymphomas by undergoing a BM biopsy. Immunohistochemical staining of the paraffin-embedded sections of BM trephine biopsies was performed in all cases and used standard techniques with monoclonal anti-CD8, CD20 and dual immunofluorscence-labelled CD3, and CD19 antibodies and also all stained with Hematoxylin and Eosin (H&E) for morphologic assessment.RESULTS:The 26 cases of NHL comprised of 14 male (54%) and 12 female patients (46%) . The median age was (57.32) year ranged from 27-85 years. There were 23 cases of B-cell cases (88.5%) and 3 cases of T-cell lineage (11.5%) of all the cases. Among all the B-cell lymphomas, 15 cases showed interstitial infiltration in the bone marrow, while among the T-cell lymphoma two cases showed diffuse infiltration. CONCLUSION:1.In these 26 cases NHL patients with marrow involvement, B cell phenotype comprised 88% of cases.2.B-cell NHLs had predominance of interstitial infiltration in bone marrow biopsies in comparison with the T-cell lymphoma, in which diffuse infiltration was predominant..