Detection of Epstein Barr Virus lmp-1 Gene in Non Hodgkin Lymphoma Iraqi Patients


Non-Hodgkin's lymphomas (NHLs) are hematologic malignancy with the highest prevalence worldwide. They are broadly classified as B-cell or T-cell lymphoma depending on which type of lymphocyte becomes cancerous, B-cell lymphoma is more common than T-cell lymphoma.The EBV infects certain epithelial cells and linked to the development of multiple cancers, including NHL, HL, and nasopharyngeal carcinoma.This study designates to determine the frequency EBV lmp-1 inNHL Iraqipatients, and todetermine the correlation between EBV lmp-1and subtypes of NHL.A 42 FFPE blocks were examined included 32 blocks from NHL patients, and 10 blocks from reactive follicular hyperplasia. Genomic DNA was extracted from these blocks using a specific kit and amplified by polymerase chain reaction (PCR) by using oligonucleotide primers specific for EBV lmp-1 gene .Our result shows EBV lmp-1 was detected in 43.8 % cases of NHL, and in 10.0% cases of reactive follicular hyperplasia with no significant (P>0.0.5) variation between these groups. In addition to that,incidence ofEBV lmp-1 was more frequently detected in high grade. From these results we conclude that EBV lmp-1 plays an important role in NHL development especially in high grade histopathological type.