Thebcl2 t(14;18) Chromosomal Translocation in Iraqi Patients with Non-Hodgkin's Lymphoma

Abstract

Non-Hodgkin lymphomas (NHLs) are hematologic malignancy with the highest prevalence worldwide. They are broadly classified as B-cell or T-cell lymphoma, depending on which type of lymphocyte becomes cancerous. Chromosomal translocations are the hallmark genetic aberration in NHL with specific translocations often selectively associated with particular NHL subtypes. An important example is the t(14;18( which leads to constitutive activation of the bcl2 oncogene by the enhancers of the immunoglobulin heavy chain locus.One of the main objectives of this study to determine the frequency of bcl2 t(14.18) chromosomal translocation in NHL Iraqi patients and also to determine the correlation of bcl2 translocation with subtypes of NHL. A 46 formalin fixed paraffin embedded blocks were examined included 32 blocks from NHL patients, in addition to 10 blocks from reactive follicular hyperplasia, and 4 tonsils as a control group. Genomic DNA was extractedand amplified by polymerase chain reaction (PCR) by using oligonucleotide specific primers for major breakpoint region (MBR), and minor cluster region (MCR) of the bcl-2 gene on chromosome 18 and conserved JH sequence on chromosome 14. The present result illustrate significantly higher frequency of bcl2 t(14,18) at MBR in NHL and reactive follicular hyperplasia Iraqi patients.We concluded the bcl2t(14,18) chromosomal translocation is a hallmark in NHL Iraqi patients with no association with specific subtype.