Effect of Hemodialysis & Dialyzer Biocompatibility on Erythrocyte Glutathione &Related Enzymes on Uremic Patients

Abstract

Twenty five healthy control and forty two end stage renal disease (ESRD) patients on regular hemodialysis treatment were enrolled in this study. Blood samples Were drown immediately before and after hemodialysis, and erythrocyte glutathione peroxidase (GSH-Px), glutathione reductase (GSSG-RD) activities, as well as reduced glutathione (GSH) concentration were measured. To study the effect of a single hemodialysis session on glutathione defense system and oxidative stress, the patients were then divided into two groups according to the dialyzer type used in the session (cuprophane, n=23 patients and polysulfone, n=19 patients). GSH-Px activity, as well as GSH concentration was significantly decreased in ESRD patients as compared with controls. GSSG-RD activity was significantly elevated in ESRD patients as compared with controls. A single hemodialysis session, regardless to the type of dialyzer used, did not induce any significant effect on any of the parameters measured. Cuprophane dialyzer did not result in any significant changes among glutathione defense system parameters. Polysulfone dialyzer exerted a significant correction on glutathione system parameters. The findings conclude that the Glutathione defense system may serve as a good index for monitoring oxidative stress and dialyzers biocompatibility in ESRD patients on regular hemodialysis treatment and the use of polysulfone rather than cuprophane dialyzers in hemodialysis procedure is recommended.