Evaluation of Genotoxicity of Cisplatin in White Mice

Abstract

The current study was conducted to evaluate genotoxic and cytotoxic effects of cisplatin in white mice.The cytogenetics end points (micronuclei) were used as genomarker to assess the genotoxicity. Mitotic index(MI) and hematological end points (PCEs, NCEs, Hb, MCV, and Ptl) were used to assess cytotoxicity. Cisplatin is a chemotherapeutic agent for many kinds of cancer. We tested the dosages 2.0 and 10 mg/kg.b.wt. by intraperitoneal ingection. Standard methods for Micronucleus were used to assess the clastogenic and aneugenic effects of cisplatin in bone-marrow cells of white mice. The Results of this study showed elevated levels of micronucleated polychromatic erythrocytes (MnPCEs), While MI decreased significantly, (PCEs, NCEs, Hb, MCV, and Ptl increased significantly. This result lead to suggest that the tested dosages of cisplatin have genotoxic and cytotoxic effects in white mice.