Antibacterial and Antibiofilm Activity of Zinc Oxide Nanoparticles on Pseudomonas aeruginosa Isolates from Diabetic Foot Infections Grade 1


Abstract:The aim of this study was to investigate antibacterial activities and disruption of biofilmstructure by zinc oxide nanoparticles (ZnO NP). Pseudomonas aeruginosa was chosen as anindicator of pathogenic because its natural resistance to antibiotics and its ability to formbiofilm on surfaces makes the cells impervious to therapeutic concentrations.Twenty one isolate were taken from patients suffering from diabetic foot ulcer grads 2infections who attended from AL-Kindy Teaching Hospital in Iraq. Different concentrationsof zinc oxide (NP) had been used (25-20000) μg/ml.The results showed that the high concentrations (500-20000) μg /ml were lethal tobacteria also the minimum inhibitory concentration (MIC) and sub (MIC) of zinc oxide npwas determined in this study. Some of the isolates were inhibit in concentration 100 μg/mland others inhibit in concentration 75 μg/ml and according to these result the sub–MIC were75 μg/ml to some isolates and 50 μg/ml to others, these concentration were inhibitor biofilmproduction.Our study indicates that zinc oxide nanoparticles could potentially be an antibacterialreagent to treat diseases caused by bacteria.