Abstract

Seasonal samples have been collected of a green algae Cladophora crispata to study its capability of accumulation and producing hydrocarbon compounds by measuring the concentration of these compounds in the collection stage (first stage) , then they cultured it in a laboratory by using Chu-10 medium during two times after (15 and 30) days to guarantee the purity of algae from hydrocarbon compounds which accumulated from the environment . The concentrations of these compounds were measured in each period to determine the accumulation from the environment and the production from the alga itself . The result were showed high concentration of compounds in the first period (directly after collecting time of the sample) which are (137.48 , 48.79 , 47.44 , and 44.44) ng/g in Spring , Summer , Autum , and Winter respectively ,and the decrease gradually at the second period (after 15 days) reaches ( 73.16 , 11.33 , 43.84 , and 15.38) ng/g and at the third period (after 30 days) reaches (52.70 , 10.65 , 30.22 , and 6.34) ng/g in the same sequence of the seasons . PAHs have been identified by capillary GC and showing a seasonal variation with high concentration were observed during Summer at the first stage 72.04 ng/g wereas low concentration during Spring after one month of sample collecting and planting (1.21) ng/g . Aliphatic compounds in alga also were determind and shows high concentrations during Winter at the first stage 186.17 ng/g wereas alow concentrations in Summer after 30 days of the collection of the sample 3.32 ng/g . Carbon Preference Index (CPI) and pristane to phytane ratio were uses to identify the origin of the hydrocarbon compounds which were found to be both biogenic and anthropogenic resources .

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The article was added to IASJ on 2015-01-25

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